Nisin | 食品添加物合法業者資訊網

由NKitagawa著作·2019·被引用7次—Bacteriocinsareantimicrobialpeptidesproducedbyvariousbacteria.NisinisatypeofbacteriocinproducedbysomestrainsofLactococcus ...

Food-grade nisin was obtained from Sigma–Aldrich (St. Louis, MO, USA). Bepridil was purchased from Enzo Life Sciences (Farmingdale, NY, USA). Fetal bovine serum (FBS) was from Biowest (Santiago, Chile). The ten-well glass slides printed with highly water-repellent marks (6 mm in diameter) were from Matsunami Glass Industry (Osaka, Japan; cat. no. TF1006).

HaCaT cells (Boukamp et al. 1988[1]) were cultured in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10 % FBS, 2 mM glutamine, 100 μg/ml streptomycin, and 100 mg/ml penicillin. This growth medium contained approximately 2.2 mM calcium. Cells were seeded at a subconfluent density (1.2 × 105 cells/cm2) in ten-well glass slides; the medium was changed the following day, and the cells were cultured for 2 days. After culturing for 2 days, cells were treated for 24 h with 23, 47, 93, or 186 μg/ml nisin in growth medium. Nisin was diluted in 0.05 % acetic acid before addition to the me...