Horseradish peroxidase | 食品添加物合法業者資訊網

Theenzymehorseradishperoxidase(HRP),foundintherootsofhorseradish,isusedextensivelyinbiochemistryapplications.Itisametalloenzymewith ...

Chemical compound and enzyme

The enzyme horseradish peroxidase (HRP), found in the roots of horseradish, is used extensively in biochemistry applications. It is a metalloenzyme with many isoforms, of which the most studied type is C. It catalyzes the oxidation of various organic substrates by hydrogen peroxide.

The structure of the enzyme was first solved by X-ray crystallography in 1997[2] and has since been solved several times with various substrates.[3] It is a large alpha-helical glycoprotein which binds heme as a redox cofactor.

Alone, the HRP enzyme, or conjugates thereof, is of little value; its presence must be made visible using a substrate that, when oxidized by HRP using hydrogen peroxide as the oxidizing agent, yields a characteristic color change that is detectable by spectrophotometric methods.[4][5]

Numerous substrates for horseradish peroxidase have been described and commercialized to exploit the desirable features of HRP. These subs...