液化澱粉芽孢桿菌WF02菌株之培養條件優化與生物防治效力評估 | 食品添加物合法業者資訊網

由施智為著作·2019—生物防治；液化澱粉芽孢桿菌；培養條件優化；回應曲面法；十字花科黑腐病；biocontrol；Bacillusamyloliquefaciens；optimizationofculture ...

Bacillus amyloliquefaciens strain WF02 was isolated from Wufeng mountain, Taichung city. It has been demonstrated that WF02 could control tomato bacteria wilt caused by Ralstonia solanacearum, as well as control bacterial fruit blotch caused by Acidovorax avenae subsp. citrulli. In my preliminary data indicated that the antagonistic activity of this bacterium was positively correlated with its biomass. Accordingly, the purpose of this study was to develop the optimal fermentation conditions that are economically viable, and that yield maximum biomass in the shortest unit of time. In the initial stage of optimization, the shake-flask culture system was used to investigate the factors associated with cell culture conditions. The molasses and ammonium nitrate were selected as the ideal carbon and nitrogen sources. The pH level ranged from 7 to 9, and the optimum culture temperature ...