Pyruvate decarboxylase | 食品添加物合法業者資訊網
由MTFlikweert著作·1996·被引用261次—InSaccharomycescerevisiae,thestructuralgenesPDC1,PDC5andPDC6eachencodeanactivepyruvatedecarboxylase.Replacementmutationsinthesegenes ...
In Saccharomyces cerevisiae, the structural genes PDC1, PDC5 and PDC6 each encode an active pyruvate decarboxylase. Replacement mutations in these genes were introduced in a homothallic wild-type strain, using the dominant marker genes APT1 and Tn5ble. A pyruvate-decarboxylase-negative (Pdc-) mutant lacking all three PDC genes exhibited a three-fold lower growth rate in complex medium with glucose than the isogenic wild-type strain. Growth in batch cultures on complex and defined media with ethanol was not impaired in Pdc- strains. Furthermore, in ethanol-limited chemostat cultures, the biomass yield of Pdc- and wild-type S. cerevisiae were identical. However, Pdc- S. cerevisiae was unable to grow in batch cultures on a defined mineral medium with glucose as the sole carbon source. When aerobic, ethanol-limited chemostat cultures (D = 0 center dot 10 h-1) were switched to a feed containing glucose as the sole carbon...
Pyruvate decarboxylase | 食品添加物合法業者資訊網
Pyruvate Decarboxylase | 食品添加物合法業者資訊網
Pyruvate decarboxylase | 食品添加物合法業者資訊網
Pyruvate decarboxylase | 食品添加物合法業者資訊網
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